Caco-2 Transfection Reagent (Colon Carcinoma Cells)

Altogen Biosystems is a recognized producer and custom research organization for the pharmaceutical industry. Custom research products and other goods include xenografts, optimization of preclinical research, stable transfection of cells, IND applications and more. Dedicated to assisting researchers with state-of-the-art and efficient services, Altogen Biosystems endeavors to further modern clinical techniques. Visit the Altogen Biosystems website for colon xenograft models .

Initially derived from human tissue by Jorgen Fogh, Caco-2 cells are human colorectal adenocarcinoma cells that can serve as transfection hosts, provided that the transfection is done with minimally-intrusive transfection reagents . The cells exhibit in vivo  characteristics even in vitro , such as the differentiation into enterocytes that are found in the small intestine. Caco-2 cells produce the correct proteins and enzymes that are found in real tissue, making them optimal candidates for initial drug research and the monitoring of cell physiology after introduced foreign DNA or other nucleic acid sequence.

Tumorigenic colon cancer cells cultured from a 72 year old Caucasian male patient with colorectal adenocarcinoma were used to develop the Caco-2 HTB-37 cell line. The Caco-2 cells are known to double in about 62 hours and have been characterized as tumorigenic, epithelial cells.The use of these cells as transfection models for human colon cancer research is faciliated by the use  of Caco-2 transfection reagents created by  Altogen Biosystems . The Caco-2 transfection reagent is a biodegradable polymer based reagent that yields high transfection efficacy while also being gentle enough to be used to analyze an individual cell.  Caco-2 Transfection Reagent (Colon Carcinoma Cells)